Summary
Type: SPE
Application #: 302970
Catalog#: 730033 - SPE Cartridges, Chromabond, NH2, 3ml, PP, 500mg, 50/pk
Detection:
Conditions: Conditioning: two column volumes of n-Hexane
Sample application: 1 ml lipid extract in n-Hexane
Washing: one column volume of n-Hexane
Elution: 1) 3 ml n-Hexane / Chloroform (9:2/v:v) for the cholesterol esters
2) 4 ml n-Hexane / Diethylether (1:1/v:v) for the triacyl glycerins
3) and 8 ml Methanol for the phopholipids.
Authors: Rickert, R.
Source: Universität Hamburg